FIG. 7.

dPER(Δ) is highly impaired in its ability to repress dCLK-CYC-mediated expression in flies. wper⁰¹; per⁺-HAHis (M16) or wper⁰¹; per(Δ)-HAHis (F21) flies were collected at the indicated times in LD. (A and B) RT-PCR assays were used to measure the relative levels of total dper or dClk RNAs. (C) ChIP assays targeting the dper circadian regulatory sequence were performed in the presence of either nonspecific antibody (GPS) or anti-dCLK antibodies (GP-47). Shown is a representative gel of amplified bands following immunoprecipitation with GPS or anti-dCLK antibodies; also shown is 0.33% of the input DNA material (left panel). Densitometry quantification of bands from hot PCR analysis was calculated as a percentage of the total input DNA, and the results from two independent experiments were averaged (right panel).