FIG. 1.

Chromosome III replicators and loss rates of ARS deletion chromosomes. (A) Positions of ARS elements are indicated above the line representing chromosome III. ARS elements that function as efficient chromosomal replication origins are indicated as black boxes with white type. ARS elements that function in less than 25% of cell cycles are boxed with dotted lines (41). ARS elements that do not normally function as replication are not boxed.CEN3 is indicated by a filled circle on the map line. (B) ARS elements were deleted from the SUP11-1-marked chromosome of strain CF4-16B33 as described in Materials and Methods, and loss rates were determined by fluctuation analysis. The numbers within the bars indicate the last one or two digits of the names of the ARS elements deleted (e.g., 7 refers to ARS307). Error bars indicate standard deviations.