FIG. 2.

Loss rates of chromosomes fragmented near ARS304, ARS310, or both. Diagrams on the left show the 0ORIΔ derivatives of chromosome III used. 0ORIΔ, 5ORIΔ, and 6ORIΔ chromosomes described for Fig. 1 were fragmented as described in Materials and Methods. pYND104 was used for fragmentations near ARS304, producing the 283-kb fragment, and pYND56 was used for fragmentations near ARS310, producing the 174-kb fragment. The 141-kb derivative was fragmented at both ends. Loss rates were determined by fluctuation analysis. Error bars indicate standard deviations.