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. 2007 Apr 23;27(13):4652–4663. doi: 10.1128/MCB.01246-06

FIG. 3.

FIG. 3.

Two-dimensional gel analysis of replication intermediates of ARS301, ARS302, ARS303/ARS320, and ARS304 in 0ORIΔ and 6ORIΔ chromosomes fragmented near ARS310. DNA was prepared from strains CB08D3 (0ORIΔ) and YDN289 (6ORIΔ), which carry the S. carlsbergensis balancer chromosome. A diagram of the restriction fragment examined is shown below each pair of two-dimensional patterns. The filled rectangle represents the position of the ARS element(s). In all cases, the left panel of a pair shows the pattern obtained from the 0ORIΔ chromosome fragment and the right panel shows the pattern obtained from the 6ORIΔ fragment. A diagram of the 174-kb 6ORIΔ chromosome fragment examined is shown at the bottom of the figure. Landmarks on the map line are labeled as described for Fig. 2.