FIG. 6.

Depletion of RINT-1 leads to abnormal Golgi dynamics during cell cycle progression. (A) Micrographs of HeLa-RINT-1i cells coimmunostained with antibodies against GM130, a Golgi apparatus marker, and phosphorylated histone 3 (H3P), which is a marker of different cell cycle stages. Cells were synchronized at the G₁/S boundary by double thymidine blocking and induced with (+Dox) or without (−Dox) 5 μg of doxycycline/ml as depicted in Fig. 4E. Pro, prophase; pro-meta, prometaphase; meta, metaphase; ana, anaphase; telo, telophase. Bars, 10 μm. (B to E) The percentages of HeLa-RINT-1i cells with the dispersed Golgi apparatus at interphase (B); mitotic Golgi blob formation at prophase (C); Golgi haze formation at prometaphase, metaphase, and anaphase (D); and the reassembled Golgi structure at telophase (E) after induction with or without 5 μg of doxycycline/ml were determined. Cells at interphase (n > 300); prophase, prometaphase, or metaphase (n > 200); anaphase (n > 50); and telophase (n > 100) were examined. All the data are representative of results from two independent experiments. Error bars, standard deviations.