FIG. 7.

Depletion of RINT-1 disrupts the pericentriolar localization of the Golgi apparatus. (A and B) Micrographs of HeLa-RINT-1i cells coimmunostained with antibodies against giantin and γ-tubulin (arrows) upon treating with (+Dox) or without (−Dox) 5 μg of doxycycline/ml. Dissociation of RINT-1 from the pericentriolar region with the dispersed Golgi structure at interphase (A) and with the nonreassembled Golgi structure at telophase (B) was observed in RINT-1-depleted cells (+Dox). Bars, 10 μm. (C) Diagram of proposed RINT-1 functions involved in maintaining centrosome integrity as well as Golgi dynamics. The depletion of RINT-1 leads to mitotic cell death as well as tumorigenesis with haploinsufficiency.