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. 2007 Apr 30;27(13):4685–4697. doi: 10.1128/MCB.02138-06

FIG. 7.

FIG. 7.

Analysis of the L1 ORF2 translation. (A) Schematic of the dicistronic L1 5′UTR-p40-Fluc mRNA used for these experiments. (B) Comparison of Fluc expression levels directed by the L1 5′UTR and the L1 5′UTR-p40 sequences and the effect of the 5′ cap on the translation of the L1 5′UTR-p40-Fluc transcript. The values of Fluc were normalized to Rluc activity originated from the cotransfected Rluc mRNA and expressed relative to the Fluc/Rluc activity of capped L1 5′UTR-p40-Fluc mRNA (taken as 1 unit). The values are shown on a logarithmic scale. The efficiency of the dicistronic RF mRNA translation is displayed for a background estimation. (C) Effect of point mutations and substitutions on the L1 5′UTR-p40-Fluc translation efficiency. The experiment was performed as described for panel B, with the exception that the Fluc/Rluc value of L1 5′UTR-p40-Fluc was taken as 100 units.