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. 2007 Apr 23;27(13):4917–4930. doi: 10.1128/MCB.01789-06

FIG. 6.

FIG. 6.

Chemical inhibition of Akt induces expression of Mxi1-SRα in DLD-1 cells. (A) DLD-1 cells were treated with 1 μg/ml triciribine or solvent (dimethyl sulfoxide [DMSO]) for 24 h and analyzed for expression of Mxi1-SRα, Akt, and FOXO3a by immunoblotting. Phosphorylation of Akt and FOXO3a was investigated using phospho-specific antibodies. (B) DL23 cells were transfected with a luciferase construct containing sequences of the Mxi1-SRα promoter, Mxi1-SRα(−441/+1292), and treated with 1 μg/ml triciribine or solvent (DMSO) for 24 h. Values were normalized to the activity of a cotransfected Renilla luciferase reporter.