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Effects of VPA and PI3K inhibition on in vivo Dictyostelium phospholipid turnover. Aggregation-competent wild-type (AX2) Dictyostelium cells were permeabilized, and lipids were labeled using [γ-32P]ATP in the presence of VPA (0.5 mM) or LY294002 (50 μM). (A) Thin-layer chromatography separation of phospholipids for two independent samples is shown. (B) Quantification of phospholipids (±standard deviation) (n = 4). **, P < 0.01; ***, P < 0.001.
