TABLE 3.
Recombinational analysis of mating of C. neoformans KN99α NAT with KN99a NEO on medium containing pigeon guanoa
| Marker combination | No. of strains (%) |
|---|---|
| α NAT | 5 (11) (parental) |
| a NEO | 7 (16) (parental) |
| α | 5 (11) |
| a | 3 (7) |
| α NEO | 7 (16) |
| a NAT | 6 (13) |
| α NAT NEO | 9 (20) |
| a NAT NEO | 3 (7) |
| Total | 45 (100) |
a
Opposite-mating-type strains of C. neoformans var. grubii genetically marked with a NAT or NEO resistance gene were washed with PBS, and equal volumes of each mating type were combined. The mixture was placed as a 10-μl drop onto medium containing 25% pigeon guano. Plates were incubated in the dark at 25°C for 7 days. Spores were microdissected, and the resulting colonies were screened for mating type and the presence of each marker.