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EAP1 expression is required for in vitro biofilm formation under flow. C. albicans strains YJB6284 (EAP1/EAP1), SPY313 (eap1Δ/EAP1), SPY314 (eap1Δ/eap1Δ), and SPY315 (eap1Δ/eap1Δ/EAP1) were cultured on the surface of a parallel plate flow chamber with YPD at 37°C flowing at 6 dyn/cm2. Cells were allowed to incubate on the surface in sodium phosphate buffer for 30 min prior to the initiation of flow.
