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Autophagy was blocked in ΔMgatg1 mutants. The vacuole was filled with autophagic bodies (A) and the cytoplasm with autophagosomes in the wild-type Guy-11 cells (B), whereas no autophagic body was evident in the vacuole (C) and no autophagosomes were evident in the cytoplasm of ΔMgatg1 mutants (D) after culturing on MM−N medium in the presence of 2 mM PMSF for 4 h. An autophagosomes are indicated by the arrow. Bars, 0.5 μm.
