TABLE 2.
Novel E. histolytica introns culled from the EST dataa
| Representative EST | Transcript | GenBank accession no. | Predicted function | 5′ Splicing site | 3′ Splicing site | BLAT coordinates | BLAT scaffold | Size | No. of unique ESTs | Effect on protein |
|---|---|---|---|---|---|---|---|---|---|---|
| Class I | ||||||||||
| EHAA254TF | 101.m00114 | ELA47515.1 | Conserved hypothetical protein | GUUUGU | AAG | 10427-10489 | 101 | 62 | 1 | Alters the C terminus |
| EHAA741TF | 42.m00181 | N/A | Pseudogene Ras family GTPase | GUUUGU | UAG | 30857-30960 | 42 | 103 | 7 | Alters the C terminus |
| EHAF244TR | 19.m00316 | ELA50600.1 | Hypothetical protein | GUUUGU | UAG | 77147-77207 | 19 | 60 | 1 | Eliminates amino acids |
| EHAA453TR | 178.m00101 | ELA45803.1 | 3′ UTR of hypothetical protein | GUUUGU | AAG | 36163-36214 | 178 | 51 | 3 | Alters the C terminus |
| EHAA547TR | 110.m00129 | ELA47260.1 | Rho family GTPase | GUUUGU | UAG | 38325-38392 | 110 | 67 | 2 | Alters the C terminus |
| EHABP41TR | 254.m00073 | ELA44597.1 | Hypothetical protein | GUUUGU | AAG | 5957-6014 | 254 | 57 | 3 | Alters the C terminus |
| EHADQ25TR | 18.m00335 | ELA50675.1 | DNA replication licensing factor | GUUUGU | UAG | 98259-98343 | 18 | 84 | 1 | Alters the C terminus |
| EHAET77TR | 18.m00328 | ELA50668.1 | Molybdopterin biosynthesis | GUUUGU | UAG | 90251-90312 | 18 | 61 | 2 | Alters the C terminus |
| EHAGK16TR | 264.m00090 | ELA44495.1 | Sec13 protein | GUAUGU | UAG | 5564-5620 | 264 | 56 | 3 | Alters the N terminus |
| EHAH331TR | 264.m00090 | ELA44495.1 | Sec13 protein | GUUUGU | UAG | 5656-5710 | 264 | 54 | 2 | Alters the N terminus |
| EHAE226TR | 133.m00132 | N/A | Hypothetical protein | GUUUGU | UAG | 16335-16395 | 133 | 60 | 1 | Alters the N and C termini |
| EHAB255TR | 52.m00167 | ELA49102.1 | Rho family GTPase | GUUUGU | UAG | 69361-69424 | 52 | 63 | 5 | Alters the C terminus |
| EHAA702TR | 231.m00059 | ELA44885.1 | Conserved hypothetical protein | GUUUGU | AAG | 7731-7834 | 231 | 103 | 8 | Alters the N terminus |
| EHAG185TRb | 47.m00184 | ELA49297.1 | Hypothetical protein | GUUUGU | AAG | 70138-70195 | 47 | 57 | 1 | Alters the N terminus |
| EHABT01TR | 57.m00155 | ELA48912.1 | Hypothetical protein | GUUCGU | UAG | 34651-34702 | 57 | 51 | 1 | Eliminates amino acids |
| EHAE044TR | 364.m00046 | ELA43561.1 | 60S ribosomal protein L27a | GUUUGU | UAG | 15314-15429 | 364 | 115 | 1 | Alters the N terminus |
| EHAET36TR | 135.m00095 | ELA46630.1 | Hypothetical protein | GUUUGU | UAG | 9119-9259 | 135 | 140 | 6 | Alters the N terminus |
| EHADY14TR | 366.m00044 | ELA43555.1 | Hypothetical protein | GUUUGU | UAG | 6738-6786 | 366 | 48 | 1 | Alters the N terminus |
| EHAG990TR | 152.m00113 | ELA46298.1 | Hypothetical protein | GUUUGU | UAG | 24791-24927 | 152 | 136 | 1 | Alters the N terminus |
| EHAAP93TR | 195.m00094 | ELA45475.1 | 60S ribosomal protein L24 | GUUUGU | UAG | 31405-31461 | 195 | 56 | 2 | Alters the N terminus |
| Class II | ||||||||||
| EHAAY54TR | 88.m00175 | ELA47893.1 | 3′ UTR of hypothetical protein | GUUUGU | UAG | 55917-56020 | 88 | 103 | 2 | N/A |
| EHAE226TR | 23.m00311 | ELA50387.1 | 3′ UTR of in Rho GTPase | GUUUGU | UAG | 24514-24574 | 23 | 60 | 1 | N/A |
| EHAES83TR | 350.m00049 | ELA43647.1 | 5′ UTR of in Rho GTPase | GUUAAG | UAG | 5995-6143 | 350 | 148 | 1 | N/A |
| EHAA378TRb | 21.m00231 | ELA50513.1 | 5′ UTR of 40S ribosomal protein S14 | GUUUGU | UAG | 17466-17539 | 21 | 73 | 3 | N/A |
| EHAA726TR | 312.m00035 | ELA43981.1 | 5′ UTR of 60S ribosomal protein L9 | GUUUGU | UAG | 6822-6956 | 312 | 134 | 12 | N/A |
| EHAHG49TR | 144.m00101 | ELA46471.1 | Similar to cap binding protein | GUUUGU | UAG | 16107-16176 | 144 | 69 | 1 | N/A |
| EHAF084TR | 39.m00252 | ELA49583.1 | Glycotransferase | GUUUGA | UAG | 80547-80602 | 39 | 55 | 1 | N/A |
| Class III | ||||||||||
| EHAAM93TR | N/A | N/A | Similar to 6.m00429 | GUUUGA | UAG | 14120-14172 | 338 | 52 | 1 | New to E. histolytica |
| EHACJ50TRb | N/A | N/A | Similar to pantothenate kinase | GUUUGU | AAG | 76031-76107 | 39 | 76 | 1 | New to E. histolytica |
| EHAFD09TR | N/A | N/A | Similar to UFD1-1 | GUUUGU | UAG | 73812-73859 | 11 | 47 | 1 | New to E. histolytica |
| EHAC353TRb | N/A | N/A | Similar to acriflavin resistance protein | GUUUGU | UAG | 4398-4451 | 389 | 53 | 2 | New to E. histolytica |
| EHAEL21TR | N/A | N/A | Similar to YIP1 Golgi protein | GUUUGU | UAG | 52191-52240 | 62 | 49 | 2 | New to E. histolytica |
| EHAEU30TR | N/A | N/A | CCCH-domain protein | GUUAGU | UAG | 89669-89734 | 5 | 65 | 2 | New to E. histolytica |
| EHACJ32TR | N/A | N/A | CCCH domain protein | GUUUGU | UAG | 90154-90220 | 5 | 66 | 2 | New to E. histolytica |
| EHAHB45TRb | N/A | N/A | No homology, novel | GUUUGU | UAG | 23491-23547 | 154 | 56 | 1 | New to E. histolytica |
a
Class I introns align by BLAT to genes that were not annotated to contain an intron in that region. Class II introns align by BLAT to the UTRs of genes. Class III introns align by BLAT to regions that were not annotated to contain genes. N/A, not applicable.
b
EST for which the spliced product has been cloned and sequenced.