FIG. 2.

A complete set of glycolytic enzymes is targeted to the cytosol. (A) RH TATi parasites were electroporated with expression plasmids which contain the ORFs of the indicated genes fused to a C-terminally located c-myc tag. The complete ORFs of the indicated genes were expressed, with the exception of phosphofructokinase I, for which only the first 500 amino acids were used. The fusion proteins were detected in stably transfected parasites by indirect immunofluorescence staining using an anti-myc antibody. (B) All the enzymes necessary for a typical glycolytic pathway are targeted to the cytosol of T. gondii. All images are at the same magnification. The bar represents 10 μm.