FIG. 3.

TPI II, PGK II, and PK II are targeted to the apicoplast. RH TATi parasites were stably tranfected with expression plasmids which contained the ORFs of the indicated genes fused to a C-terminally located c-myc tag. For TPI II and PGK II, the complete ORF of the indicated length was expressed. For PK II, the first 400 amino acids (aa) were used. Stably transfected parasites were subjected to a second transfection with pSag-ACP-YFP Cat, which encodes the apicoplast marker protein ACP-YFP. The myc-tagged fusion proteins were detected by indirect immunofluorescence staining using an anti-myc antibody. The obtained signals colocalize with the ACP-YFP signal, thus confirming the plastid localization. All images are at the same magnification. The bar represents 10 μm.