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. 1996 Nov 26;93(24):14199–14203. doi: 10.1073/pnas.93.24.14199

Figure 1.

Figure 1

Spectrophotometric assay for MTD activity in protein extracts from ELI3-2-expressing E. coli cells. Crude extract (242 μg of protein) was incubated in MTD assay buffer with (circles) or without (open circles) the cofactor NAD+. The arrow indicates the time point of addition of 0.2 units of authentic Actinobacillus MTD (solid squares).