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. 2007 Sep;18(9):3388–3397. doi: 10.1091/mbc.E07-02-0126

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© 2007 by The American Society for Cell Biology

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Figure 3. — cAMP/PKA-dependent anx 2–S100A10 complex binds cell surface CFTR. (A) Immunoblots of avidin agarose precipitates from 16HBE14o⁻ lysates ± FSK or PKI/FSK/IBMX, biotinylated for 30 min at 4°C and probed for CFTR, anx 2, and S100A10. Cell surface CFTR associates with anx 2 and S100A10. (B) Immunoblots of anx 2 immunoprecipitates from 16HBE14o⁻ lysates ± FSK or PKI/FSK/IBMX (postavidin precipitation in A) probed for CFTR, S100A10, and anx 2. Anx 2 does not associate with noncell surface CFTR. (C) Immunoblots of CFTR immunoprecipitates from 16HBE14o⁻ lysates ± FSK or PKI/FSK/IBMX (postavidin precipitation in A) probed for CFTR, S100A10, and anx 2. Noncell surface CFTR does not associate with S100A10 and anx 2. To confirm equal loading of the immunoprecipitates, blots were stripped and reprobed with anti-anx 2 (HH7; B) or anti-CFTR (R&D Systems Europe; C).