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spo9 mutant exhibits a sporulation deficiency and temperature-sensitive growth. (A) Wild type (TN8), spo9-B261 (YF2), and spo9Δ (YF16) were incubated at 28°C on sporulation medium (MEA) for 2 d. Bar, 10 μm. (B) The same strain as shown in A was streaked on complete medium (YEA) and incubated at 25 or 36°C for 3 d.
