Table 1.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| S. pombe | ||
| MKW5 (FY7456)a | h90 | Nakamura-Kubo et al. (2003) |
| TN8 (FY7132)a | h90 leu1-32 | Nakamura et al. (2001) |
| TN75 | h90/h90 ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 | Nakamura et al. (2001) |
| B261 (FY12194)a | h90 spo9-B261 ade6-M210 | Bresch et al. (1968) |
| YF2 (FY13113)a | h90 spo9-B261 leu1-32 | This study |
| YF5 | h90spo9-B261 leu1<< GFP-psy1 leu1-32 | This study |
| YF9 | h90/h90 fps1Δ::ura4+/fps1+ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 | This study |
| YF16 | h90 spo9Δ::ura4+leu1-32 ura4-D18 | This study |
| YF51 | h90leu1<< GFP-ypt7 leu1-32 | This study |
| YF54 | h90 spo9Δ::ura4+leu1<<GFP-ypt7 leu1-32 ura4-D18 | This study |
| YF69 | h90 leu1-32 ura4-D18 pAL(fps1-GFP) pDS473(spo9) | This study |
| YF75 | h90 leu1-32 ura4-D18 pTN54 pDS473(spo9) | This study |
| YF76 | h90 leu1-32 ura4-D18 | This study |
| YF77 | h90 fps1Δ::ura4+ade6 leu1-32 ura4-D18 pREP81(fps1) | This study |
| YF78 | h90 fps1Δ::ura4+ade6 leu1-32 ura4-D18 pREP81(ERG20) | This study |
| YF81 | h90 spo9Δ::ura4+leu1-32 ura4-D18 pAL(spo9) | This study |
| YN68 | h90leu1<< GFP-psy1 leu1-32 | Nakase et al. (2004) |
| S. cerevisiae | ||
| W303 (BY4502)a | MATa/α ade2-1/ade2-1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 | R. Rothstein (Columbia Univ.) |
| SFNY368 | MATα bts1Δ::URA3 ura3-52 leu2-3,112 | Jiang et al. (1995) |
| YF73 | MATa/α ERG20/erg20Δ::URA3 ade2-1/ade2-1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 | This study |
| YF79 | MATaerg20Δ::URA3 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 pYEL2(ERG20) | This study |
| YF80 | MATaerg20Δ::URA3 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 pYEL2(fps1) | This study |
| AH109 | MATaura3-52 his3-200 ade2-101 trp1-901 leu2-3,112 gal4Δ met−gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 ura3::MEL1 UAS-MEL1TATA-lacZ | Takara-Bio |
| E. coli | ||
| SF7 | ΔispA::neo Δ(srl-recA)306::Tn10 | Fujisaki et al. (2005) |
| DH5α | supE44 ΔlacU169(=80lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Sambrook et al. (1989) |
| DH10B | Δ(mrr-hsd RMS-mcrBC) mcrA recA1 | Sambrook et al. (1989) |
a The strain was obtained from the Yeast Genetic Resource Center of Japan supported by the National BioResource Project (YGRC/NBRP) (http:/yeast.lab.nig.ac.jp/nig/). The S. pombe strains constructed in this study are deposited at the YGRC/NBRP.