Table 1.
Strains used in this study
| Strain | Genotype | MAT | Background | Reference |
|---|---|---|---|---|
| yMT1448 | ura3Δ leu2Δ his3Δ | a | S288c | Brachmann et al. (1998) |
| yMT3040 | bud21::BUD21YFP-his5+ sec63::SEC63CFP-kanR ura3Δ leu2Δ his3Δ | a | S288c | This study |
| yMT3121 | sfp1Δ::URA3 bud21::BUD21YFP-his5+ sec63::SEC63CFP-kanR ura3Δ leu2Δ his3Δ | α | S288c | This study |
| yMT3131 | sch9Δ::natR bud21::BUD21YFP-his5+ sec63::SEC63CFP-kanR ura3Δ leu2Δ his3Δ | α | S288c | This study |
| yMT3160 | whi5Δ::natR sec63::SEC63CFP-kanR ura3Δ leu2Δ his3Δ | a | S288c | This study |
| yMT3182 | cln3Δ::URA3-GAL1-CLN3-1 bud21::BUD21YFP-his5+ sec63::SEC63CFP-kanR ura3Δ leu2Δ his3Δ | α | S288c | This study |
| yMT3985 | sec63::SEC63CFP-kanR crm1Δ::kanR [pDC-crm1(T539C)-LEU2/CEN] | a | W303/S288c | This study |
| yMT3981 | bud21::BUD21YFP-his5/+ sec63::SEC63CFP-kanR/+ ura3Δ leu2Δ his3Δ | a/α | S288c | |
| N-419 | ura3Δ leu2Δ his3Δ lys2Δ | a | S288c | This study |
| W303 | ade2-1 trpl-1 leu2-3,112 his3-11,15 ura3 can1-100 | a | W303 | R. Rothstein |
| BS100 | cln1::LEU2-GALpr-CLN1-HA3 cln2Δ::TRP1 cln3Δ::HIS3 ade2-1 trpl-1 leu2-3,112 his3-11,15 ura3 | a | W303 | Schneider et al. (1996) |