Figure 10.

Depletion of GSH leading to heat-induced apoptosis in cells with the cit1 deletion is caused by a shortage of glutamate. Yeast cells (wild type, Δcit1, Δcit2, and Δcit1Δcit2) grown in SCD or in SCD with 10 mM GSH, 10 mM glutamate, 10 mM cysteine, or 10 mM GSSG were incubated at 50°C. To evaluate the surviving cells, aliquots of the sample were taken at intervals, serially diluted, spotted on SCD agar plates, and incubated at 30°C for 3 d.