Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The yeast metacaspase Yca1 is activated during heat-induced death of cells with the cit1 deletion. Yeast strains (wild type, Δyca1, Δcit1, and Δcit1Δyca1) grown in SCD were exposed to heat stress (50°C; 10 min). Metacaspase activation was analyzed by dual staining with FITC-VAD-fmk and PI, followed by bivariate flow cytometry analysis. Cells detected by forward scatter were plotted by PI fluorescence on the x-axis and by FITC fluorescence on the y-axis (Wysocki and Kron, 2004).
