Figure 1.

Overexpressed GFP–Ncd colocalizes with cytoplasmic MTs and changes their intracellular distribution. Left, MT distribution visualized by Cy3 fluorescence in a control untransfected cell injected with Cy3–tubulin. Middle, GFP (middle left) and Cy3 (middle right) fluorescence in a GFP–Ncd-expressing cell injected with Cy3–tubulin. Right, merge of the two middle images (green, GFP; red, Cy3). Unlike control untransfected cells MTs in GFP–Ncd-transfected cells form thick, sparse cables that are often curved. As seen from the merged image on the right, GFP–Ncd constructs colocalizes with MTs. Bar, 10 μm.