Table 2.
Observed results of overexpression suppression analysis of Lte1 regulators in the checkpoint pathway
| Deletion | Overexpression |
|||||
|---|---|---|---|---|---|---|
| KIP2 | BUD6 | ATC1 | MSL1 | LTE1 | Control | |
| kip2Δ | NA | 2.1 | 3.1 | 2.5 | 11.0 | 8.2 |
| bud6Δ | 12.0 | NA | 1.4 | 2.3 | 11.1 | 8.6 |
| atc1Δ | 9.7 | 6.1 | NA | 2.2 | 11.6 | 9.6 |
| msl1Δ | 15.8 | 13.7 | 10.2 | NA | 15.5 | 10.7 |
| lte1Δ | 1.8 | 0.0 | 0.0 | 3.0 | NA | 2.7 |
Checkpoint integrity was assayed by counting cells with multiple GFP-TUB1—labeled spindle pole bodies, expressed here as a percentage of all multinucleate cells in asynchronous culture. n ≥ 242 in each case.