Figure 1.

Conditional activation of GyrB.PKR induces the phosphorylation and activation of Akt/PKB. (A–D) Protein extracts (50 μg) from serum-deprived HT1080 cells expressing either GyrB.PKR or GyrB.PKRK296H, untreated or treated with coumermycin (Coum; 100 ng/ml) for up to 12 or 24 h, were subjected to immunoblotting with antibodies against the indicated proteins. The ratio of phosphorylated to total protein is indicated. The ratio was set to 1 for each cell line in the absence of coumermycin treatment. The data represent one of four reproducible experiments.