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GyrB.PKR acts upstream of PI3K. (A–C) Serum-starved HT1080 cells expressing GyrB.PKR were left untreated or treated with coumermycin (100 ng/ml) in the absence or presence of (A) LY294002 (LY; 20 μM), (B) rapamycin (Rapa; 20 nM), or (C) wortmannin (Wort; 100 nM) for the indicated times. Protein extracts (50 μg) were subjected to immunoblotting with antibodies against the indicated proteins. The data represent one of three reproducible experiments.
