Skip to main content
. Author manuscript; available in PMC: 2007 Aug 24.
Published in final edited form as: Mol Endocrinol. 2006 Nov 30;21(2):524–537. doi: 10.1210/me.2006-0253

Fig. 5. Effect of PG on GnRH-Induced Gonadotropin Subunit Promoter Activities.

Fig. 5

LβT2 cells were transiently transfected with αGSU-Luc (A), LHβ-Luc (B) and FSHβ-Luc (C) promoter constructs before being pretreated for 30 min with PGE2 or PGF2α or iloprost (500 nm) followed by addition of GnRH (100 nm for 6 h). Cells were harvested and assayed for an increase in reporter gene expression expressed as relative light units (RLU), after normalization of transfection efficiency with an internal control. GnRH significantly increased all three subunits (P < 0.001, one-way ANOVA). Note that only PGF2α had a significant inhibitory effect on GnRH induction of LHβ-Luc (*, P < 0.05).