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. 2007 Jun 1;189(15):5574–5581. doi: 10.1128/JB.00564-07

FIG. 4.

FIG. 4.

Comparison of acetyl-P and acetyl-CoA pools from cells aerated at 37°C in mMOPS supplemented with 0.8% pyruvate. Samples were harvested at regular intervals, extracts were prepared, and small molecules were separated by 2D-TLC. (A) Signal ascribed to acetyl-P plotted as a percentage of the total radioactivity applied to the plate. □, WT (strain AJW678); ○, acs mutant (strain AJW1781); ▵, ackA mutant (strain AJW1939); ⋄, ackA pta mutant (strain AJW2013). (B) Signal ascribed to acetyl-CoA plotted as a percentage of the total radioactivity applied to the plate. □, WT (strain AJW678); ○, acs mutant (strain AJW1781); ▵, ackA mutant (strain AJW1939). The plotted values are the means of two independent experiments ± standard deviation. OD600, optical density at 600 nm.