FIG. 6.

Comparison of ATP (▵), acetyl-P (□), acetyl-CoA (○), and GTP (⋄) from WT cells (strain AJW678) aerated at 37°C in mMOPS supplemented with 0.8% pyruvate. Samples were harvested at regular intervals, extracts were prepared, and small molecules were separated by 2D-TLC. Signals ascribed to small molecules were plotted as percentages of the total radioactivity applied to the plate. The plotted values are the means of two independent experiments ± standard deviation. OD₆₀₀, optical density at 600 nm; phos'd cmpd, phosphorylated compound.