TABLE 3.
Transfer of SaPIbov2-tetM and chromosomal aur::ermBa
Prophage | SaPIbov2 | MC-induced phage titerb | SaPIbov2 titer (Tcr)c
|
aur::ermB titer (ErmB)d
|
||
---|---|---|---|---|---|---|
RN4220 | RN981 | RN4220 | RN981 | |||
80α | Wild type | 1.4 × 109 | 1.3 × 109 | 6.8 × 108 | 840 | 0 |
80α | Δint | 3 × 108 | 1.4 × 103 | 0 | 200 | 0 |
φ11 | Wild type | 3.2 × 109 | 5.2 × 103 | 90 | 220 | 0 |
φ11 | Δint | 8.3 × 109 | 60 | 0 | 110 | 0 |
φ147 | Wild type | 4.4 × 109 | 2.6 × 103 | 1.9 × 103 | 460 | 0 |
φ147 | Δint | 6.3 × 109 | 560 | 0 | 730 | 0 |
The data are data from a representative experiment.
Number of phage particles/ml of induced culture, using RN4220 as the recipient strain.
Number of SaPIbov2 particles/ml of induced culture, using RN4220 or RN981 (recA mutant) as the recipient strain.
Number of ErmB-resistant transductants/ml of induced culture, using RN4220 or RN981 (recA mutant) as the recipient strain.