FIG. 3.
Physical interactions between DNA polymerases and PCNA. SPR analyses were performed using a Biacore system to detect the physical interactions between PolBI and PfuPCNA and between PolD and PfuPCNA. Purified PfuPCNA was immobilized on a Biacore sensor chip, and purified PolBI or PolD was analyzed at five different concentrations, as indicated on the right side of each sensorgram. We checked separately that proteins not related to PfuPCNA do not change their sensorgrams from the baseline when this PfuPCNA-immobilized sensor chip is used. The equilibrium constant (KD) was calculated from the obtained sensorgrams.