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The PIP-box-like motifs of PolBI and PolD are essential for PfuPCNA binding. Physical interactions with PfuPCNA were compared among the wild type (WT) and the PIP-box-truncated mutant (ΔPIP) for both PolBI and PolD by using the Biacore system. Purified PfuPCNA was immobilized on a Biacore sensor chip, and purified DNA polymerases were analyzed.
