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. 2007 Jun 1;189(15):5504–5514. doi: 10.1128/JB.00518-07

FIG. 2.

FIG. 2.

Study of the effect of rulAB genes on the accumulation of Phe+ revertants on phenol-minimal plates in P. putida wild-type strain PaW85 (PaWrulAB) and in its GO repair-defective derivatives PaWMutY (mutYrulAB), PaWMutT (mutTrulAB), and PaWMutM (mutMrulAB). (A) Accumulation of stationary-phase mutations in strains PaWRulAB, PaWMutYRulAB, and PaWMutTRulAB. (B) Accumulation of stationary-phase mutations in strains PaW85RulAB and PaWMutMRulAB. Data for at least five parallel experiments are presented. In all cases, means ± standard deviations (error bars) for at least 10 plates calculated per 1 × 109 cells are shown. Differences between average accumulation rates of Phe+ mutations per day per 1 × 109 cells measured in the presence of the rulAB genes during a 3- to 10-day starvation period were not statistically significant (except for strain PaWMutYRulAB) compared to those measured in counterpart strains without the rulAB genes (P = 0.17 for PaW85; P = 0.27 for PaWMutM; P = 0.31 for PaWMutT; and P < 0.001 for PaWMutY) at the 95% confidence level, based on the Student t test.