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. 2007 May 25;189(15):5429–5440. doi: 10.1128/JB.01929-06

TABLE 2.

Genes demonstrating increased expression at 37°C

Genea Blattner no. Productb Change (n-fold) at 37°/23°Cc
Amino acid transport and metabolism
    argD b3359 Acetylornithine delta-aminotransferase 2.4
    aroF b2601 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase (DAHP synthetase), tyrosine-repressible 2.2
    aroG b0754 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase (DAHP synthetase) 2.0
    asnA b3744 Asparagine synthetase A 2.4
    carB b0033 Carbamoyl phosphate synthase, large subunit 2.8
    cysD b2752 ATP-sulfurylase, subunit 2 (ATP-sulfate adenylyltransferase) 2.2
    cysH b2762 3′-Phosphoadenosine 5′-phosphosulfate (PAPS) reductase 2.6
    cysM b2421 Cysteine synthase B (O-acetylserine sulfhydrolase B) 2.4
    gcvP b2903 Glycine cleavage complex protein P, glycine decarboxylase, PLP dependent 1.9
    hisC b2021 Histidinol phosphate aminotransferase 1.9
    hisJ b2309 Histidine transport protein (ABC superfamily, peri_bind) 2.6
    hisQ b2308 Histidine and lysine/arginine/ornithine transport system (ABC superfamily, membrane) 2.2
    idnD b4267 l-idonate 5-dehydrogenase, NAD binding 1.8
    livG b3455 ATP-binding component of high-affinity branched-chain amino acid transport system 1.9
    livK b3458 High-affinity branched-chain amino acid transport protein (ABC superfamily, peri_bind) 2.4
    lysC b4024 Aspartokinase III, lysine sensitive 2.6
    lysP b2156 Lysine-specific permease 2.5
    nanA b3225 N-Acetylneuraminate lyase (aldolase) 2.1
    tesA b0494 Acyl-coenzyme A thioesterase I; also functions as protease I 2.0
    trpD b1263 Anthranilate synthase component II 2.1
    ybiK b0828 l-asparaginase 1.9
    yecC b1917 Putative ATP-binding component of a transport system 2.5
    yecS b1918 Putative amino acid transport protein (ABC superfamily, membrane) 2.1
Carbohydrate transport and metabolism
    araA b0062 l-arabinose isomerase 1.7
    galK b0757 Galactokinase 3.3
    garD b3128 (d)-galactarate dehydrogenase 3.0
    garL b3126 Alpha-dehydro-beta-deoxy-d-glucarate aldolase 5.4
    garP b3127 Putative transport protein 6.3
    lamB b4036 Maltoporin, high-affinity receptor for maltose and maltoseoligosaccharides; phage lambda receptor 2.3
    mglA b2149 ATP-binding component of methyl-galactoside transport and galactose taxis 3.3
    mglB b2150 Galactose transport protein (ABC superfamily, peri_bind) 3.1
    mglC b2148 Methyl-galactoside transport and galactose taxis 4.4
    nagE b0679 PTS family enzyme IIC (N terminal); enzyme IIB (center); enzyme IIC (C terminal) 2.4
    sgbH b3581 3-Keto-l-gulonate 6-phosphate decarboxylase 1.8
    srlB b2704 PTS family enzyme IIA, glucitol/sorbitol specific 2.2
    uidA b1617 Beta-d-glucuronidase 2.3
    uxuB b4323 d-mannonate oxidoreductase 2.1
    yeiC b2166 Putative kinase 2.4
Cell motility and secretion
    fliQ b1949 Flagellar biosynthesis 1.9
Cell wall/membrane biogenesis
    nmpC b0553 DLP12 prophage; outer membrane porin, at locus of qsr prophage 10.0
    ompT b0565 DLP12 prophage; protease VII, outer membrane protein 3b (a), putative porin 3.3
    yciD b1256 Outer membrane protein W; colicin S4 receptor; putative transport protein 3.0
Coenzyme transport and metabolism
    btuB b3966 Outer membrane porin: vitamin B12/cobalamin transport, receptor for E colicins 2.1
    entC b0593 Isochorismate hydroxymutase 2, enterochelin biosynthesis 2.0
    folE b2153 GTP cyclohydrolase I 2.7
    ubiA b4040 4-Hydroxybenzoate-octaprenyltransferase 2.1
Defense mechanisms
    hsdR b4350 Endonuclease R, host restriction 2.8
Energy production and conversion
    fumB b4122 Fumarase B (fumarate hydratase class I), anaerobic isozyme 1.9
    galT b0758 Galactose-1-phosphate uridylyltransferase 2.6
    hybA b2996 Hydrogenase-2 small subunit 2.1
    napF b2208 Fe-S ferredoxin-type protein, electron transfer 1.7
    nirB b3365 Nitrite reductase [NAD(P)H] subunit 3.3
    sucD b0729 Succinyl-coenzyme A synthetase, alpha subunit 1.9
    ybiC b0801 Putative dehydrogenase 3.1
    yqhD b3011 Putative alcohol dehydrogenase 3.3
Inorganic-ion transport and metabolism
    cirA b2155 Outer membrane pore protein, receptor for colicin I, requires TonB 2.8
    cysA b2422 ATP-binding component of sulfate permease A protein; chromate resistance 2.3
    cysC b2750 Adenosine 5′-phosphosulfate kinase 2.7
    cysI b2763 Sulfite reductase, alpha subunit 2.2
    cysJ b2764 Sulfite reductase (NADPH), flavoprotein beta subunit 2.7
    cysN b2751 ATP-sulfurylase (ATP:sulfate adenylyltransferase), subunit 1, probably a GTPase 2.1
    cysP b2425 Thiosulfate binding protein 3.1
    fecA b4291 KpLE2 phage-like element; outer membrane porin, receptor for ferric citrate 2.5
    fecR b4292 KpLE2 phage-like element; regulator in multicomponent regulatory system with FecI/FecA 3.3
    fepA b0584 Outer membrane porin, receptor for ferric enterobactin and colicins B and D 2.0
    fepB b0592 Ferric enterobactin (enterochelin) binding protein; periplasmic component 1.9
    fepC b0588 ATP-binding component of ferric enterobactin transport 2.0
    fepG b0589 Ferric enterobactin transport protein 2.0
    fes b0585 Enterochelin esterase 2.7
    ycdO b1018 Hypothetical protein 1.8
Lipid transport and metabolism
    acs b4069 Acetyl-coenzyme A synthetase 2.4
    fadE b0221 Medium-long-chain fatty acyl-coenzyme A dehydrogenase 1.8
    garR b3125 Tartronate semialdehyde reductase (TSAR) 1.8
Nucleotide transport and metabolism
    allB b0512 Allantoinase 1.9
    rihA b0651 Pyrimidine-specific nucleoside hydrolase 2.0
    rihC b0030 Nucleoside hydrolase 3.3
Posttranslational modification, protein turnover, chaperones
    ccmC b2199 Heme export protein (ABC superfamily, membrane) 2.3
    ccmG b2195 Heme lyase/disulfide oxidoreductase (thiol-disulfide interchange protein) 1.8
    cysU b2424 Sulfate, thiosulfate transport system permease T protein 2.7
    yeeD b2012 Hypothetical protein 3.0
Replication, recombination, and repair
    nohB b0560 DLP12 prophage; bacteriophage DNA- packaging protein 2.0
    sbcB b2011 Exonuclease I, 3′→ ′-specific; deoxyribophosphodiesterase 2.0
    trs5_1 b0259 CP4-6 prophage; IS5 protein 1 2.0
    trs5_10 b3218 IS5 protein 10 2.3
    trs5_11 b3505 IS5 protein 11 2.2
    trs5_3 b0656 IS5 protein 2.2
    trs5_4 b1331 IS5 protein 2.1
    trs5_5 b1370 IS5Y transposase 2.4
    trs5_6 b1994 IS5 protein 2.4
    trs5_7 b2030 IS5 protein 2.6
    trs5_8 b2192 IS5 protein 2.5
    trs5_9 b2982 IS5 protein 3.2
Secondary-metabolite biosynthesis, transport and catabolism
    entB b0595 2,3-Dihydro-2,3-dihydroxybenzoate synthetase, isochroismatase 2.1
    entE b0594 2,3-Dihydroxybenzoate-AMP ligase 2.2
    idnO b4266 5-Keto-d-gluconate 5-reductase 2.0
    srlD b2705 Glucitol (sorbitol)-6-phosphate dehydrogenase 1.9
    yeiN b2165 Hypothetical protein 3.1
Signal transduction mechanisms
    ycdT b1025 Putative transmembrane protein 1.7
Transcription
    fecI b4293 KpLE2 phage-like element; sigma 19 factor of RNA polymerase 2.2
    lrhA b2289 NADH dehydrogenase transcriptional regulator, LysR family; modulates SprE (RssB) activity 4.4
    yijC b3963 Putative regulator (TetR/AcrR family) 2.4
General function prediction or function unknown
    yadS b0157 Putative membrane protein 2.2
    yddA b1496 Putative ABC transport system ATP-binding protein 4.8
    yeeE b2013 Putative membrane component of transport system 2.2
    yfiD b2579 Putative formate acetyltransferase 4.4
Not in COGS database
    b1172 b1172 Hypothetical protein 2.0
    b2191 b2191 Unknown CDS 2.3
    cdaR b0162 Regulator of d-galactarate, d-glucarate, and d-glycerate metabolism 1.9
    cysW b2423 Sulfate transport system permease W protein 2.6
    malM b4037 Periplasmic protein of mal regulon 2.4
    pppA b2972 Bifunctional prepilin peptidase 2.7
    srlA b2702 PTS family enzyme IIC, glucitol/sorbitol specific 2.0
    srlE b2703 PTS family enzyme IIBC, glucitol/sorbitol specific 2.5
    ybcT b0556 DLP12 prophage; bacteriophage lambda endopeptidase homolog 3.5
    ybcU b0557 DLP12 prophage; bacteriophage lambda Bor lipoprotein homolog, involved in serum resistance 3.5
    yddB b1495 Hypothetical protein 8.0
    yedO b1919 d-cysteine desulfhydrase, PLP-dependent enzyme 2.4
    ygaW b2670 Putative membrane protein 4.9
    yhcE b3217 Unknown CDS 2.1
    yijD b3964 Putative membrane protein 3.4
    yjiD b4326 Unknown CDS 2.4
    ylaC b0458 Putative membrane protein 2.1
    ymfL b1147 e14 prophage; putative negative regulator 2.3
a

Genes involved in iron utilization and/or known to be Fe2+-Fur regulated are shown in boldface.

b

Product descriptions and functional categories are based upon the E. coli K-12 COGS categorization (63). Descriptions were shortened in some instances.

c

Change is indicated as the average ratio of medians (37°C/23°C). All genes included showed a statistically significant increase at 37°C as described in Materials and Methods and based on the results from five microarrays.