FIG. 1.

Effect of type 1 and P fimbrial expression on the motility and chemotaxis of UPEC strain CFT073. Motilities of the wild type (WT) (A), CFT073 ΔfliC (flagellum-negative mutant) (B), CFT073 fim L-ON (C), CFT073 fim L-OFF (D), UPEC76 (CFT073 pap double mutant) (E), and Δfim pap (fim mutant of UPEC76) (F) in soft agar are shown. Standardized cultures of each strain were stabbed into 0.25% soft agar plates and incubated for 16 h at 30°C. (G) Black bars represent the average diameters of swimming (in mm) of triplicate motility plates. Error bars represent the standard errors of the means (SEM). (H) Chemotaxis of the wild type and the fim-locked mutants to 10 mM l-aspartate (asp). Each data point represents the log₁₀ CFU/ml per 1 μl capillary. Bars indicate the mean log₁₀ CFU/ml. Filled symbols indicate capillaries filled with attractant, while open symbols indicate capillaries filled with chemotaxis buffer only. Significant differences in motility and chemotaxis between the wild type and each strain (or control) were determined using an unpaired Student's t test with Welch correction (Instat; GraphPad). *, P < 0.0001.