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Effect of class I, II, and III flagellar gene mutations on the orientation of the type 1 fimbrial IE and expression of FimH in CFT073. Strains were grown to early exponential phase (OD600 of ∼0.30) in tryptone broth at 30°C with shaking (200 rpm). A type 1 fimbrial IE assay (A) and Western blot analysis using mouse antiserum against FimH (B) are depicted. WT, wild type.
