TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| CFT073 | Wild-type pyelonephritis isolate (O6:K2:H1) | 44 |
| CFT073 fim L-ON | CFT073 fim IE phase locked; constitutive type 1 fimbrial expression | 22 |
| CFT073 fim L-OFF | CFT073 fim IE phase locked; no type 1 fimbrial expression | 22 |
| fim L-ON ΔfimAICDFGH | CFT073 fim L-ON with deletion of fimAICDFGH | This study |
| UPEC76 | CFT073 Nalr derivative; both pap operons disrupted | 45 |
| Δfim pap | UPEC76 fimAICDFGH::aphA (Kanr) | 55 |
| ΔflhDC | CFT073 flhDC::aphA (Kanr) | This study |
| ΔflhC | CFT073 flhC::aphA (Kanr) | This study |
| ΔfliA | CFT073 fliA::aphA (Kanr) | 32 |
| ΔflgM | CFT073 flgM::aphA (Kanr) | 32 |
| ΔfliC | CFT073 fliC::aphA (Kanr) | 32 |
| ΔcheY | CFT073 cheY::aphA (Kanr) | 32 |
| F11 | Wild-type cystitis isolate (O6:H31) | 58 |
| F11 fim L-ON | F11 fim IE phase locked; constitutive type 1 fimbrial expression | 56 |
| F11 fim L-OFF | F11 fim IE phase locked; no type 1 fimbrial expression | 56 |
| K-12 | Wild-type K-12 strain MG1655 | Gift from R. Welch (6) |
| K-12 fim L-ON | K-12 strain MG1655 fim IE phase locked; constitutive type 1 fimbrial expression (AAEC554) | Gift from I. Blomfield (40) |
| K-12 fim L-OFF | K-12 strain MG1655 fim IE phase locked; no type 1 fimbrial expression (AAEC358) | Gift from I. Blomfield (40) |
| Plasmids | ||
| pBAD/Myc-HisA | Arabinose-inducible expression vector (Ampr) | Invitrogen |
| pBAD-flhDC | pBAD/Myc-HisA digested with NcoI and PmeI replacing Myc-His site with flhDC (Ampr) | This study |