TABLE 2.

Ability of P. syringae pv. syringae hrp mutants to elicit the HR in test plants

P. syringae pv. syringae strain	Genotype	Phenotype classa
		N. tabacum	N. benthamiana
61	Wild type	I	I
61-N540	hrpB::nptII	IV	IV
61-N363	ΔhrpD::nptII	IV	IV
61-N19	hrpE::TnphoA	IV*	IV
61-N491	ΔhrpF::nptII	IV*†	IV
61-N492	hrpG::nptII	II*†	I
61-N460	hrpJ::nptII	II‡	I
61-2083	hrpO::TnphoA	IV	IV
61-N534	ΔhrpP::nptII	IV	II
61-N526	ΔhrpQ::nptII	IV	IV
61-N402	ΔhrpT::nptII	III*†	II
61-N407	ΔhrpV::nptII	I†	I
61-N393	ΔhrcC::nptII	IV*	IV

^aPhenotype classes: I, wild type HR; II, HR slightly reduced and/or delayed; III, HR weak and spotty, some leaves with no response; IV, no HR. Mutants deficient in hrpB, hrpD, hrpJ, hrpO, hrpP, and hrpQ were restored to wild-type ability to elicit the HR in N. tabacum by the complementing clones shown in Fig. 1. Results are based on at least four independent tests for each strain. *, The ability of the mutant to secrete HrpZ1 in culture was previously tested (24); †, similar HR phenotypes in N. tabacum were previously reported for these mutants (11); ‡, a similar HR phenotype in N. tabacum was reported for a P. syringae pv. tomato DC3000 hrpJ mutant (16).