TABLE 2.
β-Galactosidase activity of E. coli SU202 isolates expressing wild-type and mutant MexR and PA3719 proteins in the LexA-based two-hybrid system
| Two-hybrid plasmid combinationa
|
β-Galactosidase activity (Miller units)b | |
|---|---|---|
| pDP804 derivative | pMS604 derivative | |
| pDP804 | pMS604 | 535 |
| pDP804 | mexR WT | 5,856 |
| PA3719 WT | pMS604 | 7,987 |
| PA3719 WT | mexR WT | 54 |
| PA3719 WT | mexR L35P | 2,699 |
| PA3719 WT | mexR I104F | 2,769 |
| PA3719 WT | mexR M112T | 2,778 |
| PA3719 WT | mexR L135F | 2,509 |
| PA3719 WT | mexR L28P | 2,499 |
| PA3719 WT | mexR L75P | 3,312 |
| PA3719 W45A | mexR WT | 3,359 |
| PA3719 L36P | mexR WT | 1,804 |
| PA3719 S25-Y53 | mexR WT | 23 |
a
WT, wild type.
b
The data are representative of at least three independent determinations.