FIG. 3.
The lack of AcrAHI negatively affects the expression and FM accessibility of AcrBHI-Cys. (A) AcrBHI-Cys variants were produced in E. coli AG100AX (ΔacrAB ΔacrEF) in the presence and absence of AcrAHI and analyzed for FM accessibility as described in the legend of Fig. 1 and in Materials and Methods. (B) Fluorescence intensities of AcrBHI-Cys variants shown in panel A are normalized as described in the legend of Fig. 2. Error bars are standard deviations (n = 3). HiAcrB, AcrBHI; amt, amount.