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HPLC analysis of PG monomers generated by MBP-AtlA. Radiolabeled PG monomers generated by MBP-AtlA (A), generated by hen egg white lysozyme (B), and released from growing gonococci (C) were purified and analyzed by reversed-phase HPLC. Fractions were collected and analyzed for radioactivity. The fraction of radioactivity in each fraction per total radioactivity eluted was plotted over time. G, N-acetylglucosamine; M, N-acetylmuramic acid; (anh)M, 1,6-anhydro N-acetylmuramic acid.
