FIG. 2.

Morphological effects of inhibiting FtsZ, MreB, or PBP 2. E. coli MG1655 or AV23-1 was treated to inhibit one or more of the following proteins: FtsZ was inhibited by inducing SulA from pFAD38 by adding 0.2% arabinose, MreB was inhibited by adding A22 at the indicated concentrations, and PBP 2 was inhibited by adding amdinocillin (2 μg/ml). (A) AV23-1, with inhibition of both FtsZ and MreB (A22, 5 μg/ml). (B) AV23-1. Conditions were as described above (A), but A22 was removed before a shift to growth at 42°C. (C) MG1655, with inhibition of MreB (A22, 5 μg/ml). (D) AV23-1, with inhibition of MreB (A22, 5 μg/ml). (E) AV23-1, with inhibition of both FtsZ and MreB (A22, 2 μg/ml). (F) AV23-1, with inhibition of FtsZ and PBP 2.