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Effect of inhibiting MreB in a preformed spiral cell. Spiral cells were induced by inhibiting FtsZ in AV23-1 by expressing SulA from pFAD38. The cells were transferred onto agar wells containing A22 (5 μg/ml) (to inhibit MreB) and 0.2% arabinose (to inhibit FtsZ) and incubated at 42°C on a heated microscope stage. Individual spiral cells were selected for microscopic observation, and one typical experiment is shown. Numbers refer to minutes of incubation.
