TABLE 3.

Murein composition of wild-type E. coli and a PBP mutant^a

Composition	Mol%
	MG1655				AV23-1
	+FtsZ, +MreB	−FtsZ, +MreB	+FtsZ, −MreB	−FtsZ, −MreB	+FtsZ, +MreB	−FtsZ, +MreB	+FtsZ, −MreB	−FtsZ, −MreB
Pentapeptide					22.0	29.5	8.1	10.6
Monomers	69.5	69.3	64.0	61.9	63.4	65.4	59.9	60.0
Dimers	27.8	28.6	31.8	32.9	33.8	32.5	35.8	34.5
Trimers	1.5	1.2	3.6	4.0	2.3	1.7	3.6	4.5
Lipoprotein	9.4	8.2	11.8	10.5	5.4	4.5	9.2	10.2
Anhydro	2.0	1.9	4.7	5.3	1.9	1.1	4.5	4.8
DAP-DAP	2.9	2.8	5.9	7.3	2.0	1.2	4.0	5.1

^aPeptidoglycan from E. coli strains MG1655 (parental) and AV23-1 (ΔPBPs 5 and 7) was isolated and treated as described in Materials and Methods. The FtsZ-dependent increases in pentapeptide levels were consistent in two independent experiments and four independent samples (not shown).