TABLE 1.
Plasmids used in this study
| Plasmid | Gene producta | Antibiotic resistanceb | Source or reference |
|---|---|---|---|
| pBR322 | Δb | Ap | New England Biolabs |
| pTAM37 | bhis | Cm | 15 |
| pTAM46 | bV5 | Ap | 15 |
| pSBC56 | bE39-D53, his | Cm | This study |
| pSBC57 | b′E39-D53, V5 | Ap | This study |
| pSBC58 | bL54-I86, his | Cm | This study |
| pSBC76 | b′L54-I86, V5 | Ap | This study |
| pSBC97 | bE39-I86, his | Cm | This study |
| pSBC98 | b′E39-I86, V5 | Ap | This study |
| pSBC94 | bL54-A107, his | Cm | This study |
| pSBC79 | b′L54-A107, V5 | Ap | This study |
| pSBC95 | bE39-A107, his | Cm | This study |
| pSBC96 | b′E39-A107, V5 | Ap | This study |
a
his, six-histidine epitope tag at the amino terminus; V5, epitope tag with the sequence GKPIPNPLLGLDS appended to the carboxyl terminus. The replaced regions are designated by the first and last E. coli amino acids that were replaced with T. elongatus sequence.
b
Ap, ampicillin; Cm, chloramphenicol.