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. 2007 May 25;189(15):5463–5471. doi: 10.1128/JB.00191-07

TABLE 4.

Proton pumping rates of membranes prepared from cells expressing chimeric E39-to-I86 subunits

Strain/plasmid(s) Gene product(s) Final fluorescence (% of initial fluorescence) Initial rate of decrease (%/min)
KM2/pTAM37/pTAM46 bhis/bV5 22.2 ± 0.1 66.9 ± 0.6
KM2/pBR322 Δb 91.7 ± 0.5 0.5 ± 0.2
KM2/pSBC97 bE39-I86, his 34.5 ± 0.8 27 ± 2
KM2/pSBC98 bE39-I86, V5 76.0 ± 0.4 6.1 ± 1.1
KM2/pSBC97/pSBC98 bE39-I86, his/ bE39-I86, V5 28.0 ± 0.5 48 ± 3