TABLE 3.
rpoB mutation spectra of N. meningitidis H44/76 wild-type and DNA repair-deficient strainsa
| H44/76 strain genotype | Nucleotide change(s) |
|---|---|
| Wild typeb | GC → AT(103 × 3) |
| fpg | GC → AT(92 × 3) |
| mutS | GC → AT(103 × 2); GC → CG(104 × 1) |
| dinB | GC → AT(92 × 1, 103 × 1, 119 × 1) |
| uvrA | GC → AT(92 × 1, 103 × 1, 119 × 1) |
| recA6c | GC → AT(92 × 2) |
| mutS dinB | GC → AT(92 × 1, 103 × 1); GC → CG(104 × 1) |
| mutS recA6 | GC → AT(92 × 1, 103 × 1); GC → CG(104 × 1) |
| mutS uvrA | GC → AT(92 × 1, 103 × 2) |
| fpg uvrA | GC → AT(92 × 2); GC → TA(103 × 1) |
| dinB uvrA | GC → AT(103 × 3) |
| dinB recA6 | GC → AT(119 × 1); GC → CG(104 × 1); GC → TA(103 × 1) |
| recA6 uvrA | GC → AT(103 × 3) |
| mutYb | GC → TA(92 × 1, 103 × 1, 119 × 1) |
| mutY fpg | GC → TA(92 × 1, 103 × 2) |
| mutY mutS | GC → TA(103 × 3) |
| mutY dinB | GC → TA(103 × 3) |
| mutY uvrA | GC → TA(92 × 1, 103 × 2) |
| mutY recA6 | GC → TA(64 × 1, 103 × 2) |
a
Three independent rifampin-resistant strains of the MC H44/76 wild-type and DNA repair-deficient strains listed in Table 1 were analyzed for nucleotide changes conferring rifampin resistance. Subscripts indicate the nucleotide being changed (where nucleotide 1 corresponds to the first nucleotide in the sequencing primer) and the number of strains (× n) with the given transition/transversion.
b
Previously shown by Davidsen et al. (10).
c
One strain showed no nucleotide changes inside the sequenced area.