FIG. 6.
Suppression of growth and viability defects of the yofA mutant upon induction of FtsW. (A) Effect of FtsW induction on growth. The growth of ZL014 (Pspac-ftsW) cells in liquid LB medium in the absence of IPTG (×) or in the presence of the following concentrations of IPTG was determined: 0.001 mM (▪), 0.01 mM (⋄), 0.1 mM (▴), and 1 mM (□). (B) Effect of FtsW induction on growth of yofA mutants: growth of wild-type 168 (○) and ZL001 (yofA::neo) (•) cells and of ZL015 (yofA::neo Pspac-ftsW) cells in liquid LB medium in the absence (▵) or presence of 1 mM IPTG (⋄). (C) Effect of FtsW induction on colony formation of yofA mutants: CFU/ml for wild-type 168 (○), ZL001 (yofA::neo) (•), ZL014 (Pspac-ftsW) in the presence of 1 mM IPTG (▪), and ZL015 (yofA::neo Pspac-ftsW) supplemented with 1 mM IPTG (▴). The data represent the means of three independent experiments, and mean OD600 values are shown.