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RT-PCR analysis of expression of yofA and ftsW: time course of expression of yofA (A), ftsW (B), and rpsR (C). RNA (0.5 μg) was isolated at the indicated time points from LB medium cultures of wild-type strain 168 and then analyzed by RT-PCR as described in Material and Methods. The rpsR transcript encoding ribosomal protein S18 served as a control for fluctuations in total RNA amounts. The arrowheads indicate the positions of the expected RT-PCR products.
