TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype, phenotype, and relevant characteristics | Source, reference, or constructiona |
|---|---|---|
| E. coli JM105 | supE endA sbcB15 hsdR4 rpsL thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZΔM15] | 45 |
| B. subtilis strains | ||
| 168 | trpC2 | Laboratory stock |
| ZL001 | trpC2 yofA::pUCN192 | This study |
| ZL002 | trpC2 yofA::pMUTinyofA (Pspac-yofA) | This study |
| ZL003 | trpC2 thrC:: pTCCyofA (PyofA-yofA) | This study |
| ZL004 | trpC2 yofA:: pUCN192 thrC::pTCCyofA | ZL001 → ZL003 |
| ZL005 | trpC2 thrC::pTCCZ1 (ftsAZ-lacZ) | This study |
| ZL006 | trpC2 thrC::pTCCZ2 (divIB-lacZ) | This study |
| ZL007 | trpC2 thrC::pTCCZ3 (divIC-lacZ) | This study |
| ZL008 | trpC2 thrC::pTCCZ4 (ftsL-pbpB-lacZ) | This study |
| ZL009 | trpC2 thrC::pTCCZ5 (rodA-lacZ) | This study |
| ZL010 | trpC2 thrC::pTCCZ6 (ftsW-lacZ) | This study |
| ZL011 | trpC2 yofA::pUCN192 thrC::pTCCZ6 | ZL001 → ZL010 |
| ZL012 | trpC2 Pxyl-yofA (multicopy yofA) | This study |
| ZL013 | trpC2 Pxyl-yofA thrC::pTCCZ6 | ZL012 → ZL010 |
| ZL014 | trpC2 ftsW::pMUTinftsW (Pspac-ftsW) | This study |
| ZL015 | trpC2 yofA::pUCN192 ftsW::pMUTinftsW | ZL001 → ZL014 |
| ZL016 | trpC2 yofA::pUCN192 thrC::pTCCZ1 | ZL001 → ZL005 |
| ZL017 | trpC2 yofA::pUCN192 thrC::pTCCZ2 | ZL001 → ZL006 |
| ZL018 | trpC2 yofA::pUCN192 thrC::pTCCZ3 | ZL001 → ZL007 |
| ZL019 | trpC2 yofA::pUCN192 thrC::pTCCZ4 | ZL001 → ZL008 |
| ZL020 | trpC2 yofA::pUCN192 thrC::pTCCZ5 | ZL001 → ZL009 |
| Plasmids | ||
| pMUTinT3 | Vector carrying bla erm | 35 |
| pMUTinyofA | pMUTinT3 carrying Pspac-yofA | This study |
| pMUTinftsW | pMUTinT3 carrying Pspac-ftsW | This study |
| pUCN192 | Vector carrying bla neo | 20 |
| pUCNyofA | pUCN192 carrying an internal region of yofA gene | This study |
| pTCC1 | Integration vector at thrC carrying bla cat | 24 |
| pMF20 | Vector carrying bla cat | 34 |
| pHY300PLK | Vector carrying bla tet | 25 |
| pTCCZ | pTCC1 carrying the coding region of lacZ gene | This study |
| pTCCZ1 | pTCCZ carrying the upstream region of ftsA (ftsAZ-lacZ) | This study |
| pTCCZ2 | pTCCZ carrying the upstream region of divIB (divIB-lacZ) | This study |
| pTCCZ3 | pTCCZ carrying the upstream region of divIC (divIC-lacZ) | This study |
| pTCCZ4 | pTCCZ carrying the upstream region of ftsL (ftsL-pbpB-lacZ) | This study |
| pTCCZ5 | pTCCZ carrying the upstream region of rodA (rodA-lacZ) | This study |
| pTCCZ6 | pTCCZ carrying the upstream region of ftsW (ftsW-lacZ) | This study |
| pTCCyofA | pTCC1 carrying native yofA | This study |
| pHYXY1 | Vector carrying bla tet | This study |
| pHYXYyofA | pHYXY1 carrying the entire yofA open reading frame | This study |
a
Arrows indicate transformation from donor DNA to recipient strain.